A gold nanoparticle-modified PVC/TTF-TCNQ composite amperometric biosensor for glucose determination
Sánchez, G., Cano, M., ,Avila, JL., Mayen, M., Mena, ML., Pingarron, M., Rodríguez, R.
Abstract
A comparison of the analytical performances of several enzyme biosensor designs, based on the use of different tailored gold nanoparticle-modified composite PVC/TTF-TCNQ electrodes, is discussed. The analytical characteristics of glucose calibration plots and kinetic parameters of the enzyme reaction were compared for the biosensors tested: Aucoll + GOx, GOx + Aucoll, Mixed GOx + Aucoll and PVC/TTF-TCNQ-Aucoll. The presence of this nanomaterial enhances the analytical performance with respect to the precursor biosensor without Au. The proposed biosensor can be applied in batch (response linear up to 2.0 mM, sensitivity of 45 ± 0.5 mA M−1 with a limit of detection, s/n = 3, of 6.2 × 10−6 M) and in FIA systems (linear range between 0.1 and 8 mM, sensitivity of 3.67 ± 0.3 mA M−1 with a limit of detection, s/n = 3, of 1.9 × 10−5 M).
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