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There are organizations for re-homing animals that are also specialized in re-homing dogs and cats who have been research subjects. Why can’t we do this for more animals and especially for the rats that are used extensively in research and they are not intended to be pets as they are not as common as pets? Even though previous research highlights neonatal handling as an essential process for rearing less stressed and emotionally sensitive adult rats, there is very little information about a specific neonatal handling schedule that could be a crucial factor in promoting their welfare and adoption as pets. The aim of this study is to propose a standardized handling schedule assessment based on implementation of a neonatal handling procedure that mimics maternal behavior, in order to have a positive effect on the rats’ welfare, decreasing their stress responsiveness towards humans and promoting their adoption as pets. DA rats (DA/OlaHsd) were handled during postnatal days 1 (D1) to 21 (D21) (experimental group, n=15) using a handling schedule that imitates rat maternal behavior or received only gold standard neonatal handling (control group, n=14). On D22, blinded researchers evaluated the behaviour of both groups according to their responses to handling. Blinding was used, as to try to eliminate biased results, as to examine the rats' behavior against strangers which is a crucial factor for their suitability as pets. The data were collected through a customized assessment form. The statistical analysis of both groups’ cumulative responses showed that the experimental group had less stressful responses compared to the control group (Mann-Whitney (U) test: U= 13; P < 0.01). This neonatal handling schedule may improve rat welfare during their life in the lab. Also, it could be a method that assists laboratory rats to be adopted as pets after their laboratory life.
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2. Barker, S. B., Rogers, C. S., Turner, J. W., Karpf, A. S., & Suthers-McCabe, H. M. (2003). Benefits of interacting with companion animals: a bibliography of articles published in refereed journals during the past 5 years. American Behavioral Scientist, 47(1), 94–99. https://doi.org/10.1177/0002764203255215
3. IAHAIO White Paper (2014). The IAHAIO Definitions for Animal-Assisted Intervention and Guidelines for Wellness of Animals Involved. Retrieved from http://www.iahaio.org
4. Fine, A. H. (Ed.). (2015). Handbook on animal-assisted therapy: Foundations and guidelines for animal-assisted interventions, 4th ed. San Diego, CA: Elsevier Academic Press.
5. Chandler, C. (Ed.). (2012). Animal Assisted Therapy in Counseling. New York: Routledge.
6. Davidson RJ & McEwen BS. (2012). Social influences on neuroplasticity: Stress and interventions to promote well-being. Nature Neuroscience, 15: 689–695. https://doi.org/10.1038/nn.3093
7. Huot, R.L., Plotsky, P.M., Lenox, R.H. & McNamara, R.K. (2002) Neonatal maternal separation reduces hippocampal mossy fiber density in adult Long Evans rats. Brain Res. 950, 52–63. http://dx.doi.org/10.1016/S0006-8993(02)02985-2
8. Mirescu C., Peters J.D., Gould E. (2004) Early life experience alters response of adult neurogenesis to stress. Nat Neurosci. Aug;7(8):841-6. https://doi.org/10.1038/nn1290
9. Rees S.L. and Fleming A.S. (2001) How early maternal separation and juvenile experience with pups affect maternal behavior and emotionality in adult postpartum rats. Learn Behav., Aug 29(3):221–233. http://dx.doi.org/10.3758/BF03192910
10. Tang, A.C. , Akers , K.G. , Reeb , B.C. et al . (2006) Programming social, cognitive, and neuroendocrine development by early exposure to novelty. Proceedings of the National Academy of Sciences, USA, 103, 15716 – 15721. https://doi.org/10.1073/pnas.0607374103
11. Escorihuela, R.M., Tobeña, A., Fernández-Teruel, A. (1995) Environmental Enrichment and Postnatal Handling Prevent Spatial Learning Deficits in Aged Hypoemotional (Roman High-avoidance) and Hyperemotional (Roman Low-avoidance) Rats, Learning & Memory 2(1):40-48. http://dx.doi.org/10.1101/lm.2.1.40
12. Flecknell P (2002) Replacement, reduction and refinement. Altex 19: 73–78.
13. Ladd C.O., Huot R.L., Thrivikraman K.V., Nemeroff C.B., Meaney M.J., Plotsky P.M. (2000). Long-term behavioral and neuroendocrine adaptations to adverse early experience. Prog Brain Res 122:81-103. PMID: 10737052
14. Colorado, R.A., Shumake, J., Conejo, N. M., Gonzalez-Pardo, H., Gonzalez-Lima, F. (2006) Effects of maternal separation, early handling, and standard facility rearing on orienting and impulsive behavior of adolescent rats, Behavioural Processes, 71, 51–58. http://dx.doi.org/10.1016/j.beproc.2005.09.007
15. Raineki C., Lutz M.L., Sebben V., Ribeiro R.A., Lucion A.B. (2013) Neonatal handling induces deficits in infant mother preference and adult partner preference. Developmental Psychobiology, 55: 496–507. https://doi.org/10.1002/dev.21053
16. Fernández-Teruel, A., Escorihuela, R. M., Castellano, B., González, B., Tobeña, A. (1997) Neonatal Handling and Environmental Enrichment Effects on Emotionality, Novelty/Reward Seeking, and Age-Related Cognitive and Hippocampal Impairments: Focus on the Roman Rat Lines, Behav Genet. Nov;27(6):513-26. http://dx.doi.org/10.1023/A:1021400830503
17. van Erp, Annemoon, M.M., Kruk, Menno R., Meelis, Wout., Willekens-Bramer, Daniella C. (1994) Effect of environmental stressors on time course, variability and form of self-grooming in the rat: handling, social contact, defeat, novelty, restraint and fur moistening, Behavioural Brain Research 65 47-55. https://doi.org/10.1016/0166-4328(94)90072-8
18. Gomes, C.M., Raineki, C., Ramos de Paula P., Severino, G.S., Helena, C.V., Anselmo-Franci, J.A., Franci, C.R., Sanvitto, G.L., Lucion, A.B. (2005) Neonatal handling and reproductive function in female rats. J Endocrinol. Feb;184(2):435-45. http://dx.doi.org/10.1677/joe.1.05907
19. Whishaw, I.Q. and Kolb, B. (2005) The behaviour of the laboratory rat- A handbook with tests., Chapter 27: 287- 297; Rees SL, Lovic V, Fleming AS: Maternal behaviour. Oxford University Press. https://doi.org/10.1093/acprof:oso/9780195162851.003.0027
20. Squire L.R., Dede A.J. (2015) Conscious and unconscious memory systems. Cold Spring Harb Perspect Biol. Mar 2;7(3):a021667. https://doi.org/10.1101/cshperspect.a021667
21. Theeuwes J. (2013) Feature-based attention: It is all bottom-up priming. Phil Trans R Soc B Biol Sci 368: 20130055. https://doi.org/10.1098/rstb.2013.0055
22. Moscarello J.M., LeDoux J.E. (2013) Active avoidance learning requires prefrontal suppression of amygdala-mediated defensive reactions. J Neurosci 33: 3815–3823. https://doi.org/10.1523/JNEUROSCI.2596-12.2013.
23. Valleé, M., W. Mayo, et al. (1997) "Prenatal Stress Induces High Anxiety and Postnatal Handling Induces Low Anxiety in Adult Offspring: Correlation with Stress- Induced Corticosterone Secretion." The Jounal of Neuroscience 17(7): 2626-2636. https://doi.org/10.1523/JNEUROSCI.17-07-02626.1997
24. Hughes R.N. (2004). The value of spontaneous alternation behavior (SAB) as a test of retention in pharmacological investigations of memory. Neurosci Biobehav Rev 28(5): 497-505. http://dx.doi.org/10.1016/j.neubiorev.2004.06.006